3rd cascading phase

In the 3rd cascading phase MACRO CASCADE will develop a variety of efficient and robust carbohydrate active enzymes, with a range of specificities, for processing macroalgal polysaccharides. The resulting products will be evaluated by industry partners in the project.

They will be used for complete or selective degradation of polysaccharides

  1. during the pre-process fractionation stage requiring robust salt tolerant enzymes for reducing viscosity,
  2. to ease the extraction of small molecule, bioactive polyphenols or flavorants,
  3. for production of monosugars and/or oligosaccharide mixtures very early in the technology chain,
  4. for complete or partial targeted degradation marine polysaccharides at a later stage for manufacture of a of pure monosugars and specialty products.

Targeted enzymatic derivatives of alginate from brown algae include:

Oligosaccharides as feed components, (less viscosity and health promoting effects)

Purified high quality alginate-oligosaccharides as food supplements.

Alginate oligosaccharides enriched in guluronate-blocks for feed (and or food) – antibiotic effect – health promoting

Targeted enzymatic derivatives of laminarin from Brown algae include:

Potent health promoting branched laminari oligosachcharides that can be used in functional food products without causing drastic changes in texture of the product (e.g. beverages) in contrast to the viscous β-glucan polysaccharides.

Complete break-down of laminarin to glucosein silage fermentation

Targeted prebiotic oligosaccharides by fragmentation and enzymatic glycosylation of polysaccharides from Red and Green algae.

Natural oligosaccharides  such as galacto-oligosaccharides (GOS), fructo-oligosaccharides (FOS), mannan oligosaccharides (MOS), and recently xylooligosaccharides (XOS) show prebiotic properties.  Major application sectors include food and beverages, but animal feed has not yet been fully developed.