Deliverables

All deliverables produced in the MACRO CASCADE project will be listed below.

D1.1 Based on the selective breeding of the selected species identification of the best combination of growth characteristics and content of value-added compounds (HOR; M36).

D1.2 Report on seasonal variation of value-added components in different types of macroalgae (ORF; M36).

D1.3 Demonstration of a durable and efficient harvesting system for large scale seaweed cultivation (HOR; M42).

D1.4 Review of environmental and legislative aspects of large-scale seaweed cultivation (COS; M48).

D2.1 Report of optimized conditioning process for seaweed (DTI; M18).

D2.2 Report on drying and semi drying algae biomass for storage of algae biomass for valorisation high-value-added products (DTI; M24).

D2.3 Report on biological and chemical ensiling techniques for storage of algae biomass for valorisation medium with value-added products (DTI; M27).

D2.4 Report detailing the results of long-term storage (DTI; M36).

D2.5 Report evaluating the feasibility of collecting the algae-juice during conditioning and pre-treatment (DTI; M36).

D3.1 Process of conversion of algae into nutritious feed additives with increased (25%) digestibility (FEX; M18).

D3.2 Process for production of algae enriched rapeseed-based feed for pigs (DTI; M24).

D3.3 Process for algae-based gut – health promoting (pre and probiotic effects) animal feed additives (DTU; M46).

D3.4 Report on process development for algal based food product (FEX; M20).

D3.5 Report on clinical effect on patients with selected chronic inflammatory bowel diseases, clinical and paraclinical response obtained from patient intake of algae-based food products (SIL; M41).

D4.1 Report on biorefinery approach based on sample composition (WR; M24).

D4.2 Protocol for production of alginate-rich fraction (WR; M36).

D4.3 Protocol for production of fucoidan-rich fraction (WR; M36).

D4.4 Protocol for production of mannitol and laminarin (WR; M24).

D4.5 Protocol for production of protein-rich fraction (WR; M36).

D4.6 Protocol for production of polyphenol (LUN; M36).

D5.1 Protocol on conversion of laminarin (from WP4) polysaccharides into branched immunomodulating β-glucan oligosaccharides (MATIS; M44).

D5.2 Protocol on the development of biocatalytic processes for conversion of alginate (from WP4) into unsaturated uronic acids for chemical industry (MATIS; M47).

D5.3 Protocol on the development of biocatalytic processes for conversion of fucoidan (from WP4) into bioactive fucoidan oligosaccharides (DTU; M40).

D5.4 Information on composition, and structure properties of macroalgal products of the consortium (WR; M45).

D6.1 Report defining the specific scope of the sustainability assessment, including identifying sustainability indicators and defining the scenarios to be assessed based on the production processes forming part of the other Work Packages (TNO; M12).

D6.2 Report with the techno economic analysis (TNO; M28).

D6.3 Report with the Social Aspects (ORF; M36).

D6.4 Evaluation of the environmental impact of the Macro Cascade scenarios (TNO; M36).

D6.5 Integrated sustainability assessment, providing the pros and cons and limitations of MacroCascade indicators and scenarios (TNO; M46).

D6.6 Business Model Canvas results and comparative SWOT and QSPM analyses for full exploitation of the project results (ORF; M46).

D7.1 Project online presence (incl. project website, newsletter, sciences blog and social media) (ORF; M4) Public.

D7.2 MACRO CASCADE dissemination, exploitation and IPR strategy paper (ORF; M6).

D7.3 Stakeholder engagement events and results’ evaluation reports (incl. inter-project meetings, Focus Group, Citizen Panel and MACRO CASCADE conferences) (ORF; M24) Public.

D7.4 Knowledge and data repository provided to relevant European platforms (incl. standards, norms, certifications and regulations relevant for MACRO CASCADE) (ORF; M12) Public.

D8.1 Project Management Collaborative Workspace (DTI; M4).

In the following an explanation about content, status, responsibility and dissemination level and is given for each deliverable:

D1.1 Based on the selective breeding of the selected species identification of the best combination of growth characteristics and content of value-added compounds (HOR; M36).

MacroCascade D1.1 – Summary

Status: Ready

Responsible partner: Hortimare

Dissemination level: Confidential

D1.2 Report on seasonal variation of value-added components in different types of macroalgae (ORF; M36).

MacroCascade D1.2 – Summary

Status: Ready

Responsible partner: Ocean Rainforest

Dissemination level: Confidential

D1.3 Demonstration of a durable and efficient harvesting system for large scale seaweed cultivation (HOR; M42).

Status: in progress (deadline March 2020)

Responsible partner: Hortimare

Dissemination level: Confidential

D1.4 Review of environmental and legislative aspects of large-scale seaweed cultivation (COS; M48).

Status: in progress (deadline September 2020)

Responsible partner: Eurofins

Dissemination level: Confidential

D2.1 Report of optimized conditioning process for seaweed (DTI; M18).

MacroCascade D2.1 – Summary

Status: Ready

Responsible partner: Danish Technological Institute

Dissemination level: Confidential

For more information contact Lars Nicolaisen: bjerager@mail.tele.dk.

D2.2 Report on drying and semi drying algae biomass for storage of algae biomass for valorisation high-value-added products (DTI; M24).

MacroCascade D2.2 – Summary

Status: Ready

Responsible partner: Danish Technological Institute

Dissemination level: Confidential

D2.3 Report on biological and chemical ensiling techniques for storage of algae biomass for valorisation medium with value-added products (DTI; M27).

MacroCascade D2.3 – Summary

Status: Ready

Responsible partner: Danish Technological Institute

Dissemination level: Confidential

For more information contact Nicolaj Ma: nima@teknologisk.dk.

D2.4 Report detailing the results of long-term storage (DTI; M36).

MacroCascade D2.4 – Summary

Status: Ready

Responsible partner: Danish Technological Institute

Dissemination level: Confidential

D2.5 Report evaluating the feasibility of collecting the algae-juice during conditioning and pre-treatment (DTI; M36).

MacroCascade D2.5 – Summary

Status: Ready

Responsible partner: Danish Technological Institute

Dissemination level: Confidential

D3.1 Process of conversion of algae into nutritious feed additives with increased (25%) digestibility (FEX; M18).

MacroCascade D3.1 – Summary

Status: Ready

Responsible partner: Fermentationexperts A/S

Dissemination level: Confidential

For more information contact Jens Legarth: jel@fexp.eu

D3.2 Process for production of algae enriched rapeseed-based feed for pigs (DTI; M24).

MacroCascade D3.2 – Summary

Status: Ready

Responsible partner: Danish Technological Institute

Dissemination level: Confidential

For more information contact Anne-Belinda Bjerre: anbj@teknologisk.dk.

D3.3 Process for algae-based gut – health promoting (pre and probiotic effects) animal feed additives (DTU; M46).

Status: in progress (deadline July 2020)

Responsible partner: The Technical University of Denmark

Dissemination level: Confidential

D3.4 Report on process development for algal based food product (FEX; M20).

MacroCascade D3.4 – Summary

Status: Ready

Responsible partner: Fermentationexperts A/S

Dissemination level: Confidential

D3.5 Report on clinical effect on patients with selected chronic inflammatory bowel diseases, clinical and paraclinical response obtained from patient intake of algae-based food products (SIL; M41).

Summary: This deliverable 3.4 describes the process of fermenting rapeseed and seaweed in food grade. In addition, we investigated the potential of such fermented food product to reduce the inflammation response in human cells. Our hypothesis was that by fermenting seaweed and rapeseed meal with selected lactic acid bacteria, the composite of naturally present anti-inflammatory compounds will be enhanced and be able to reduce the inflammatory response in human cells significantly. For this purpose, we used markers for antigenic immune stimulation after addition of lipopolysaccharide (LPS) mature dendritic cells. Different solvents were tested to extract the bioactive compounds from the food grade fermented seaweed-rapeseed. The extracts were investigated using the ratio of interleukin secretion IL-12p40/IL-10. In parallel, bioactive composition was screened with GC-MS. This analysis found a vast array of phenolic compounds associated with beneficial metabolic activity in abundance (total phenolic compounds of interest 600 ± 85 mg/kg product). Furthermore, several long chain omega-3 polyunsaturated fatty acids (PUFA) were found in abundance. The inflammation study revealed a positive effect on the anti-inflammatory response as judged by the cytokine IL-12p40/IL-10 ratio. Extracts with 80% methanol and methanol:dichlormethane shown a statistically significant effect (p < 0.05). Furthermore, the positive effect significantly increased with increasing dose of fermented rapeseed-seaweed. This effect was repeatedly consistent (n = 8) in the two batches tested, extracted with 80% methanol. We conclude that lacto-fermentation of rapeseed-seaweed has the potential to be used as a food supplement to aid reduction of inflammation. However, a clinical study with patients with a chronic inflammation disease should be evaluated as a second step. For more information contact Kjærulff Søren: skj@fermbiotics.com.

Status: in progress (deadline May 2020)

Responsible partner: Regionshospitalet Silkeborg

Dissemination level: Confidential

D4.1 Report on biorefinery approach based on sample composition (WR; M24).

MacroCascade D4.1 – Summary

Status: Ready

Responsible partner: Wageningen University & Research

Dissemination level: Confidential

For more information contact Paulien Harmsen: paulien.harmsen@wur.nl.

D4.2 Protocol for production of alginate-rich fraction (WR; M36).

MacroCascade D4.2 -Summary

Status: Ready

Responsible partner: Wageningen University & Research

Dissemination level: Confidential

D4.3 Protocol for production of fucoidan-rich fraction (WR; M36).

MacroCascade D4.3 – Summary

Status: in progress (deadline September 2019)

Responsible partner: Wageningen University & Research

Dissemination level: Confidential

D4.4 Protocol for production of mannitol and laminarin (WR; M24).

MacroCascade D4.4 – Summary

Status: Ready

Responsible partner:

Dissemination level: Confidential

For more information contact Paulien Harmsen: paulien.harmsen@wur.nl.

D4.5 Protocol for production of protein-rich fraction (WR; M36).

MacroCascade D4.5 – Summary

Status: Ready

Responsible partner: Wageningen University & Research

Dissemination level: Confidential

D4.6 Protocol for production of polyphenol (LUN; M36).

MacroCascade D4.6 – Summary

Status: Ready

Responsible partner: Lund University

Dissemination level: Confidential

D5.1 Protocol on conversion of laminarin (from WP4) polysaccharides into branched immunomodulating β-glucan oligosaccharides (MATIS; M44).

Status: in progress (deadline June 2020)

Responsible partner: Matis

Dissemination level: Confidential

D5.2 Protocol on the development of biocatalytic processes for conversion of alginate (from WP4) into unsaturated uronic acids for chemical industry (MATIS; M47).

Status: in progress (deadline September 2020)

Responsible partner: Matis

Dissemination level: Confidential

D5.3 Protocol on the development of biocatalytic processes for conversion of fucoidan (from WP4) into bioactive fucoidan oligosaccharides (DTU; M40).

Status: in progress (deadline February 2020)

Responsible partner: The Technical University of Denmark

Dissemination level: Confidential

D5.4 Information on composition, and structure properties of macroalgal products of the consortium (WR; M45).

Status: in progress (deadline July 2020)

Responsible partner: Wageningen University & Research

Dissemination level: Confidential

D6.1 Report defining the specific scope of the sustainability assessment, including identifying sustainability indicators and defining the scenarios to be assessed based on the production processes forming part of the other Work Packages (TNO; M12).

MacroCascade D6.1 – Summary

Status: Ready

Responsible partner: Energy Research Centre of the Netherlands

Dissemination level: Confidential

For more information contact Jan Wilco Dijkstra: jan_wilco.dijkstra@tno.nl.

D6.2 Report with the techno economic analysis (TNO; M28).

MacroCascade D6.2 – Summary

Status: Ready

Responsible partner: TNO

Dissemination level: Confidential

D6.3 Report with the Social Aspects (ORF; M36).

Status: in progress (deadline September 2019)

Responsible partner: Ocean Rainforest

Dissemination level: Confidential

D6.4 Evaluation of the environmental impact of the Macro Cascade scenarios (TNO; M36).

Status: in progress (deadline September 2019)

Responsible partner: TNO

Dissemination level: Confidential

D6.5 Integrated sustainability assessment, providing the pros and cons and limitations of MacroCascade indicators and scenarios (TNO; M46).

Status: in progress (deadline July 2020)

Responsible partner: TNO

Dissemination level: Confidential

D6.6 Business Model Canvas results and comparative SWOT and QSPM analyses for full exploitation of the project results (ORF; M46).

Status: in progress (deadline July 2020)

Responsible partner: Ocean Rainforest

Dissemination level: Confidential

D7.1 Project online presence (incl. project website, newsletter, sciences blog and social media) (ORF; M4).

MacroCascade D7.1 – Summary

Status: Ready

Responsible partner: Ocean Rainforest

Dissemination level: Public

For more information contact Ólavur Gregersen: olavur@oceanrainforest.com.

D7.2 MACRO CASCADE dissemination, exploitation and IPR strategy paper (ORF; M6).

MacroCascade D7.2 – Summary

Status: Ready

Responsible partner: Ocean Rainforest

Dissemination level: Confidential

For more information contact Ólavur Gregersen: olavur@oceanrainforest.com.

D7.3 Stakeholder engagement events and results’ evaluation reports (incl. inter-project meetings, Focus Group, Citizen Panel and MACRO CASCADE conferences) (ORF; M24).

Status: Delayed

Responsible partner: Ocean Rainforest

Dissemination level: Confidential

For more information contact Ólavur Gregersen: olavur@oceanrainforest.com.

D7.4 Knowledge and data repository provided to relevant European platforms (incl. standards, norms, certifications and regulations relevant for MACRO CASCADE) (ORF; M12).

MacroCascade D7.4 – Summary

Status: Ready

Responsible partner: Ocean Rainforest

Dissemination level: Public

For more information contact Ólavur Gregersen: olavur@oceanrainforest.com.

D8.1 Project Management Collaborative Workspace (DTI; M4).

Status: Ready

Responsible partner: Danish Technological Institute

Dissemination level: Confidential

For more information contact Anne-Belinda Bjerre: anbj@teknologisk.dk.

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